The first cases of H5N1 highly pathogenic avian influenza in Indiana since 2016 have been identified at a Dubois County turkey farm, but no cases of human infection have been reported.

The agency did not identify the farm.

The Indiana State Board of Animal Health said samples were collected from the farm Feb. 7 after about 100 birds died and other birds appeared lethargic and drank less water.

The samples were sent to the Indiana Animal Disease Diagnostic Laboratory at Purdue University, which said tests indicated the birds were likely infected with the virus. More samples were sent to the U.S. Department of Agriculture’s National Veterinary Services Lab in Iowa, which confirmed the presence of the H5N1 virus.

According to the BOAH, the 29,000 turkeys in the flock were culled to prevent the spread of the virus. A 10-kilometer control area was established around the farm, and commercial poultry flocks from 18 farms within the control area will be quarantined.

BOAH said it was reaching out to known hobby and backyard poultry owners in the control area to schedule testing for their birds.

The agency is asking hobby poultry owners to report illness or death to the USDA Health Birds Hotline at 866-536-7593.

Indiana ranks third in the nation for turkey production.

According to the World Organization for Animal Health, the H5N1 virus has spread over 40 countries over the last six months.